{"id":4638,"date":"2024-01-02T11:48:24","date_gmt":"2024-01-02T16:48:24","guid":{"rendered":"https:\/\/cqfa.quebec\/?p=4638"},"modified":"2024-01-02T11:52:08","modified_gmt":"2024-01-02T16:52:08","slug":"automated-fabrication-of-a-scalable-heart-on-a-chip-device-by-3d-printing-of-thermoplastic-elastomer-nanocomposite-and-hot-embossing","status":"publish","type":"post","link":"https:\/\/cqfa.quebec\/en\/automated-fabrication-of-a-scalable-heart-on-a-chip-device-by-3d-printing-of-thermoplastic-elastomer-nanocomposite-and-hot-embossing\/","title":{"rendered":"Automated fabrication of a scalable heart-on-a-chip device by 3D printing of thermoplastic elastomer nanocomposite and hot embossing"},"content":{"rendered":"<p><em>Wu, Q.; Xue, R.; Zhao, Y.; Ramsay, K.; Yan Wang, E.; Savoji, H.; Veres, T.; Cartmell, S.H.; Radisic, M. (2024). Automated fabrication of a scalable heart-on-a-chip device by 3D printing of thermoplastic elastomer nanocomposite and hot embossing. Bioactive Material. 2024, vol. 33 (46-60).<\/em><\/p>\n<p>&nbsp;<\/p>\n<p>The successful translation of organ-on-a-chip devices requires the development of an automated workflow for\u00a0device fabrication, which is challenged by the need for precise deposition of multiple classes of materials in micro-meter scaled configurations. Many current heart-on-a-chip devices are produced manually, requiring the expertise and dexterity of skilled operators. Here, we devised an automated and scalable fabrication method to engineer a Biowire II multiwell platform to generate human iPSC-derived cardiac tissues. This high-throughput heart-on-a-chip platform incorporated fluorescent nanocomposite microwires as force sensors, produced from\u00a0quantum dots\u00a0and\u00a0thermoplastic elastomer, and 3D printed on top of a\u00a0polystyrene\u00a0tissue culture base patterned by hot embossing. An array of\u00a0<em>built-in<\/em>\u00a0carbon electrodes was embedded in a single step into the base, flanking the microwells on both sides. The facile and rapid\u00a03D printing\u00a0approach efficiently and seamlessly scaled up the Biowire II system from an 8-well chip to a 24-well and a 96-well format, resulting in an increase of platform fabrication efficiency by 17,5000\u201369,000% per well. The device&#8217;s compatibility with long-term electrical stimulation in each well facilitated the targeted generation of mature human iPSC-derived cardiac tissues, evident through a positive force-frequency relationship, post-rest potentiation, and well-aligned sarcomeric apparatus. This system&#8217;s ease of use and its capacity to gauge drug responses in matured cardiac tissue make it a powerful and reliable platform for rapid preclinical drug screening and development.<\/p>\n<p>&nbsp;<\/p>\n<p class=\"link-btn-style btn-yellow\"><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2452199X23003341\" target=\"_blank\" rel=\"noopener\">Read the publication<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Wu, Q.; Xue, R.; Zhao, Y.; Ramsay, K.; Yan Wang, E.; Savoji, H.; Veres, T.; Cartmell, S.H.; Radisic, M. (2024). Automated fabrication of a scalable heart-on-a-chip device by 3D printing [&hellip;]<\/p>\n","protected":false},"author":80,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[43],"tags":[],"class_list":["post-4638","post","type-post","status-publish","format-standard","hentry","category-publications-academiques-quebecoises"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.3 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Automated fabrication of a scalable heart-on-a-chip device by 3D printing of thermoplastic elastomer nanocomposite and hot embossing - CQFA - Carrefour qu\u00e9b\u00e9cois de la fabrication additive<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cqfa.quebec\/en\/automated-fabrication-of-a-scalable-heart-on-a-chip-device-by-3d-printing-of-thermoplastic-elastomer-nanocomposite-and-hot-embossing\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Automated fabrication of a scalable heart-on-a-chip device by 3D printing of thermoplastic elastomer nanocomposite and hot embossing - CQFA - Carrefour qu\u00e9b\u00e9cois de la fabrication additive\" \/>\n<meta property=\"og:description\" content=\"Wu, Q.; Xue, R.; Zhao, Y.; Ramsay, K.; Yan Wang, E.; Savoji, H.; Veres, T.; Cartmell, S.H.; Radisic, M. 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